Identifying the proteases and their inhibitors in vivo is an essential step in understanding their clinical relevance. For such analyses, we annotated the human and mouse protease genomes [1]. The CLIP-CHIP™ is a complete protease and protease inhibitor DNA microarray chip for human and mouse, and includes 70-mer oligonucleotide probes for all 1561 human and murine proteases, inactive homologues and inhibitors [2,3]. [read more …]
The CLIP-CHIP is freely available for research collaborations. Please contact Dr. Overall for additional information.
- Puente XS, Sánchez LM, Overall CM, López-Otín C. Human and mouse proteases: a comparative genomic approach. Nat. Rev. Genet. 2003;4(7):544-558.
- Kappelhoff R, Auf dem Keller U, Overall CM. Analysis of the degradome with the CLIP-CHIP microarray. Methods Mol. Biol. 2010;622:175-93.
- Kappelhoff R, Overall C. The CLIP-CHIP oligonucleotide microarray: dedicated array for analysis of all protease, nonproteolytic homolog, and inhibitor gene transcripts in human and mouse. Curr. Protoc. Protein Sci. 2007 Aug; Chapter 21:Unit 21.19.